Journal: BMC Endocrine Disorders

Article Title: The precursor for nerve growth factor (proNGF) is not a serum or biopsy-rinse biomarker for thyroid cancer diagnosis

doi: 10.1186/s12902-019-0457-1

Figure Lengend Snippet: ProNGF serum levels after thyroidectomy and half-life. a Change in serum proNGF following total thyroidectomy . Pre- and post- thyroidectomy serum samples were available for 11 cases where pre-operative serum proNGF was detectable. No significant difference was detected between pre- and post- thyroidectomy levels of proNGF. b ProNGF in vitro half-life. Aliquots of serum negative for proNGF was spiked with 20 ng/mL recombinant proNGF dissolved in Assay Diluent A (Biosensis, Australia) in a 1:1 ratio, then incubated at 37 °C for increments of 24 h, then assayed at 1:20 dilution with Heterophilic Blocking Antibody (BL-003-1000). An exponential decay curve was fitted, giving an estimated in-vitro half-life in serum of 1.5 h. Similar results were obtained using phosphate-buffered-saline as diluent

Article Snippet: A : Effect of heterophilic antibody blockers (Ab) (BL-003-1000, Biosensis, Australia) on rate of positivity of serum proNGF levels.

Techniques: In Vitro, Recombinant, Incubation, Blocking Assay, Saline

Journal: STAR Protocols

Article Title: Protocol for SUM-PAINT spatial proteomic imaging generating neuronal architecture maps in rat hippocampal neurons

doi: 10.1016/j.xpro.2025.103637

Figure Lengend Snippet:

Article Snippet: Multiplexing blocker mouse , NanoTag Biotechnologies , Cat#K0102-50.

Techniques: Recombinant, Multiplexing, Electron Microscopy, Modification, Sequencing, Software, Cell Culture, Inverted Microscopy